HHV capsid portal protein

HHV U_L-6 (U_L-6) is a protein found in the capsid of Herpes simplex virus. U_L-6 is the protein from which the capsid portal is comprised.

"U_L-6" is nomenclature meaning that the protein is genetically encoded by the sixth (6th) open reading frame found in the viral genome segment named "Unique-Long (U_L)".

Details

Research performed in 2004 used electron microscopy to predict that U_L-6 forms 11, 12, 13, and 14-unit polymers. The dodecameric (twelve-unit) form was found to be most likely.^[1]

Refinements to the electron microscopy in 2007 allowed finding that the portal is a twelve (12)-unit polymer present at one of the twelve capsid vertices instead of the U_L-19 pentamer found at non-portal vertices.^[2]

The DNA exits the capsid in a single linear segment. DNA exit may be controlled by U_L-6 and dependent on temperature or environmental proteins. ^[3]

Assembly of portal units is an initial step in constructing capsids of viral progeny. Capsids assembled in the absence of portals lack portals.^[4]

Assembly of capsid with portal depends on interaction of U_L-6 with "scaffolding" protein U_L-26.5, amino acids 143 through 151. ^[5]

Notes

^ Trus BL, Cheng N, Newcomb WW, Homa FL, Brown JC, Steven AC (2004 Nov). "Structure and polymorphism of the UL6 portal protein of herpes simplex virus type 1". Journal of Virology. 78 (22): 12668-71. PMID 15507654. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link)(Article: [1])
^ Cardone G, Winkler DC, Trus BL, Cheng N, Heuser JE, Newcomb WW, Brown JC, Steven AC (2007 May 10). "Visualization of the herpes simplex virus portal in situ by cryo-electron tomography". Virology. 361 (2): 426-34. PMID 17188319. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link) (Article: [2])
^ Newcomb WW, Booy FP, Brown JC (2007 May 13). "Uncoating the Herpes Simplex Virus Genome". Journal of molecular biology. PMID 17540405. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link)
^ Newcomb WW, Homa FL, Brown JC (2005 Aug). "Involvement of the portal at an early step in herpes simplex virus capsid assembly". Journal of Virology. 79 (16): 10540-6. PMID 16051846. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link)
^ Singer GP, Newcomb WW, Thomsen DR, Homa FL, Brown JC (2007 May 10. Epub 2006 Dec 22). "Identification of a region in the herpes simplex virus scaffolding protein required for interaction with the portal". Journal of Virology. 361 (2): 426-34. PMID 15596809. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link) (Article: [3])

[pmid15507654-1] Trus BL, Cheng N, Newcomb WW, Homa FL, Brown JC, Steven AC (2004 Nov). "Structure and polymorphism of the UL6 portal protein of herpes simplex virus type 1". Journal of Virology. 78 (22): 12668-71. PMID 15507654. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link)(Article: [1])

[pmid17188319-2] Cardone G, Winkler DC, Trus BL, Cheng N, Heuser JE, Newcomb WW, Brown JC, Steven AC (2007 May 10). "Visualization of the herpes simplex virus portal in situ by cryo-electron tomography". Virology. 361 (2): 426-34. PMID 17188319. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link) (Article: [2])

[pmid17540405-3] Newcomb WW, Booy FP, Brown JC (2007 May 13). "Uncoating the Herpes Simplex Virus Genome". Journal of molecular biology. PMID 17540405. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link)

[pmid16051846-4] Newcomb WW, Homa FL, Brown JC (2005 Aug). "Involvement of the portal at an early step in herpes simplex virus capsid assembly". Journal of Virology. 79 (16): 10540-6. PMID 16051846. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link)

[pmid15596809-5] Singer GP, Newcomb WW, Thomsen DR, Homa FL, Brown JC (2007 May 10. Epub 2006 Dec 22). "Identification of a region in the herpes simplex virus scaffolding protein required for interaction with the portal". Journal of Virology. 361 (2): 426-34. PMID 15596809. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link) (Article: [3])

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