(at around 2h) When Robert and Sophie are in the depths of Roslyn Chapel they lift a carpet to reveal a trap door. As they lift the carpet the fleur-de-lis is clearly visible, in the next scene it is obscured by debris and needs to be cleaned out to be recognizable.
When Sophie is being held at knife-point by Silas, you see blood trickling from the cut the knife point makes. When the scene shifts back, there is no blood or cut. When it shifts again, the cut and blood are back, and she has the cut/healing wound for the rest of the film.
When Fache shows Langdon the hidden writings with the UV lamp, there are only the Fibonacci's suite and the Latin quote. Later in the bathroom, Sophie Neveu tells him Fache erased the last line which was "P.S. find Robert Langdon" and shows him a picture with the complete message.
Later, when they both come back to the murder's scene, Neveu and Langdon are trying to decipher the hidden message using the UV lamp. For a short moment, over Langdon's shoulder we are able to see the line Fache has supposedly erased.
At Sir Leigh Teabing's house in the morning - the time of day changes many times. (As seen by the sky and sun rise.)
(at around 1h 30 mins) When the plane is taxiing on the ground in England, from the view in the cockpit it is clear that the taxiway ahead dead ends in a field with woods to the right. From the outside view the plane is shown going straight through the intersection past the signalman, then turning right into a hangar.
Vinegar or scientifically known as acetic acid is a weak acid and thus unable to dissolve a sturdy material such as papyrus. Even a strong acid, like hydrochloride acid, will takes hours to dissolve papyrus. So actually they can just force open the cryptex and the vinegar-soaked papyrus would still be intact and readable.
Teabing suggests DNA testing of the body of Mary Magdalene can be used to prove Sophie is a descendent of Jesus. Not only would that not prove a link to Jesus, but there is no actual evidence that the body inside the sarcophagus is even Mary Magdalene. No genetic material exists to compare it against (and if there was the body wouldn't be needed anyway), so it could be anyone.
As Teabing, Langdon, and Sophie are arriving at the airport in England, several English police vehicles are shown on their way to intercept them. The sirens sounding on the police cars are those of American police vehicles. English police sirens sound much different than American ones.
The bottle of 1976 wine offered to Manuel Aringarosa (Alftred Molina) reads Bordeaux Supérieure at the top, but with the Languedoc appellation of origin. Bordeaux is at the southwest of France and Languedoc is at the southeast. Furthermore, Languedoc was not recognized as an AOC until 1975, after which it was allowed to be displayed on labels as Coteaux du Languedoc, never as Languedoc generically.
(at around 12 mins) When flying in the private jet and taking a call from Silas, the Bishop Aringarosa is using a cellular (GSM) phone. At this height, confirmed by a shot through the window revealing the clouds canopy below, it is impossible to get mobile network coverage.
(at around 4 mins) In Langdon's opening presentation on symbology, he shows a series of slides of modern symbols and their ancient origins. The CND "peace sign" logo is shown followed by an inverted crucifix. In fact the CND logo was created in the 1950s in Britain, by superimposing the two semaphore symbols for "N" and "D", to stand for "nuclear disarmament". The false "broken cross" history of the symbol was invented in the 1970s in the United States - suggesting Langdon didn't do his research properly. However, Langdon's presentation doesn't just speak of the intended meaning of symbols, but also of the (often presumptuous) interpretation of them, making the CND slide particularly relevant.
(at around 49 mins) The cryptex was said to be made by Leonardo Da Vinci, and the clue to open it refers to Isaac Newton's grave. Da Vinci (1452-1519) died 124 years before Newton (1643-1727) was even born. However, Sophie says that the cryptex was only designed by Da Vinci, not made by him. In the novel, Sophie states that this cryptex was built by her grandfather.
Teabing is utterly convinced that DNA testing of Mary Magdalene's body will prove that Sophie is her descendant (and by default a descendant of Jesus Christ). However, although human bones can last for thousands of years, DNA deteriorates, and without knowing how Mary Magdalene died or what state her body was buried in, it would be entirely possible that a valid sample could not be extracted from the body. An educated man who had devoted his life's work to the cause should have known that, and mentioned it in his explanations to Sophie and Langdon.
Since Sophie is repeatedly referred to as 'the LAST living descendant of Jesus Christ', her biological grandmother must therefore not be of the bloodline herself. So how did she find out about it? When Sophie's parents and brother were killed in the car crash, the cover-up story was that Sophie died in it too. Sauniere is seen carrying Sophie away from the crash site, so how did her grandmother find out that Sophie was still alive? Surely, notifying an outsider (even her own grandmother) of both of Sophie's existence AND of her bloodline, would be a huge security risk and defeat the point of the elaborate cover story and Sauniere adopting her? It makes more sense that the grandmother (who was not in the car and therefore did not see that Sophie survived) would be told the same cover-up story as the newspapers were. There's no reason why Sophie's biological grandmother could not also have been a member of the Priory (and therefore not an outsider), and therefore informed of the truth of the car crash by Sauniere.
The movie implies Sophie's family are the only direct decedents of Jesus. Family trees can branch extensively after only a handful of generations. Given that there has been almost two thousands years it's highly likely that Jesus would have many living decedents, possibly even thousands. Genetically speaking any one of them would serve the purpose of proving a link. Family trees *can* branch extensively, but may also fade out as generations may only produce one or two offspring. Also any known descendants were hunted down and killed by the church over the years.
Obvious fake 'bald spot' on the priest's head at 1:11'53" (use of a wig).
At the beginning of the movie Robert is on a conference about symbols. The first pictures he shows to the audience is a hood that the audience interprets as "racism", "hatred" or "Ku Klux Klan" but Robert says they would disagree in Spain where there are robes worn by priests. No priests dress that way in Spain, those are called "nazarenos" (nazarenes) and are laypersons who dress like that in the street processions.
At 59m 59s the falling statue does not sound like rock falling. Sounds more like an empty wooden box being hit, or falling onto a stage.
(at around 1h 11 mins) The Dome of the Rock is in the background as Mary Magdalene is leaving Jerusalem. The Dome of the Rock was built in the 7th century AD, about 600 years later.
(at around 1h 40 mins) On the bus, the mobile phone Langdon uses to look up A. Pope is a Sony Ericsson W850i, a model which had not debuted in the UK until well after May 2006.
At 34'20", the junkie's mouth (or rather jaw) does not move when he says "okay".
(at around 55 mins) When Fache visits Andre Vernet in the hospital, he calls him Vernet Andre at the beginning of his interrogation - putting his last name first. Then he doesn't speak his name again. The English subtitles has Fache start into his interrogation using Andre Vernet's name (used correctly this time) later on in the discussion - not at the beginning.
When Langdon and Sophie go into Westminster Abbey there are crowds of people, with police behind barriers and watching filming.
(at around 50 mins) As Robert and Sophie travel in the back of the armored van to Leigh Teabing's residence, they hold hands in an attempt to calm Robert due to his claustrophobia. As they hold hands, an intermittent yellow light from an external source (supposedly street lamps) is seen. It would be impossible to see any such light from within the back of an armored van. It has also been revealed that they are traveling along a secluded country track with no street lighting.
(at around 1h 35 mins) When Langdon, Teabing and Sophie are exiting the car to the church in London, the reflection of the jimmy jib operator is clearly seen on the cars windshield.
When Robert Langdon, having just escaped from the gunfire at Temple Church, says he needs to get to a library, he and Sophie Neveu are on Fleet Street, around 3-5 minutes walk from the Maughan Library, a huge university library which would have certainly provided any information Robert would require. However, instead they board a bus headed to Chelsea Library, which Robert laments is at least 30 minutes away.
Sophie presses the tracking device he's found in his pocket into a (white) bar of soap he's taken from the toilets and throws it out of the window into the back of a truck. The Louvre toilets are supplied with large lemon-shaped (and lemon-scented... and lemon-colored) soaps fixed to metal rods over the sinks. The Louvre toilets do not have windows at all.
When Robert Langdon is talking to Sophie Neveu in the Louvre toilets (0:20) there is an electric hand-drier on the wall behind her, which is connected to a British square fused electric outlet. These are not used in France.
There was no need to know the capsule's combination in order to open it as vinegar is easily frozen in a household freezer. Simply freeze the capsule and then smash it open to reveal the internal message.
The cut of the initial sequence of the movie (intercuts between the Louvre murder and Langdon's lecture), as well as the fact that the police officers come for Langdon during his post-lecture signing of books, create a major continuity lapse in the police' reasoning that Langdon is suspect. How could he commit a murder in Louvre (which can be easily timed due to the victim activating the alarm) while giving a lecture to thousand people? (In the book, he is visited by police several hours after the lecture, which would then allow him enough time to possibly commit the crime, as he is suspected.)
If Sophie's grandfather Jacques knew that the holy grail is safely kept at Louvre and no one except Sophie's grandmother (who even Sophie or the Teacher didn't know about, the Teacher even didn't know Sophie was Jacques' granddaughter) knew about it, why did he ask Sophie to find Langdon? If he wanted Sophie to know about her lineage and/or holy grail, her grandmother who herself is a Priory of Sion member could explain it better than Langdon. There is no danger either to Sophie or Sophie's grandmother or to the holy grail. Silas could never get the holy grail with the information Jacques gave and it was just a red herring. If he wanted Sophie to find her family or her grandmother, all he had to do was do nothing. Her grandmother who knew about Sophie would find her anyway after things calmed down.
(at around 1h 45 mins) When Silas shoots the Bishop, there is a shot from behind Silas where he bends down. You can see the shape of the mike battery pack under just above the rope tied around his waist to help support his robes.
(at around 7 mins) While conversing with Arigarosa in Latin, Silas calls "Paris" "Parisi". The Latin name for Paris was Lutetia; "Parisi" was the name of its inhabitants.
(at around 1h 7 mins) Sir Leigh Teabing refers to the phenomenon of peoples' minds interpreting the same thing differently ("seeing what it wants to see") as scotoma. In reality, it's called pareidolia. Scotoma is the natural "blind spot" inherent the eyes of most mammals with good vision.
(at around 1h 2 mins) Teabing says that in 325 AD Constantine "unified Rome under a single religion: Christianity". This statement is false. Constantine granted numerous privileges to the Christians, but it was not until 380, under the rule of the emperor Theodosius I, that Christianity became the official religion.
(at around 1h 2 mins) When Teabing explained the situation of Rome at the time of Emperor Constantine to Sophie, he mentioned that "Three centuries earlier, a young Jew name Jesus had come along, preaching love and a single God." This statement is false, Jesus did not preach a single God. Jesus did his ministry only among the Jews in Israel, who already believe in a single God. It was Jesus' apostles, Peter and Paul, who later came to Rome and preached Jesus' gospel and a single God to the Romans who still worshiped many gods.
(at around 1h 4 mins) Leonardo Da Vinci's The Last Supper is referred to as a fresco. Leonardo painted The Last Supper on DRY and not wet plaster, so it is not a true fresco. Because a fresco cannot be modified as the artist works, Leonardo instead chose to seal the stone wall with a layer of pitch, gesso and mastic, then paint onto it with tempera. Because it is not a true fresco, it cannot be moved easily, and this fact has caused it much deterioration and damages over the years. Furthermore, as it has received so much restoration, it is impossible to read as much into the detail of the painting as the narrative implies, as the level of detail concerned is more restoration than Da Vinci's original.