Wikipedia

Cyclin-dependent kinase 1

Cyclin-dependent kinase 1 also known as CDK1 or cell division cycle protein 2 homolog is a highly conserved protein that functions as a serine/threonine protein kinase, and is a key player in cell cycle regulation.[5] It has been highly studied in the budding yeast S. cerevisiae, and the fission yeast S. pombe, where it is encoded by genes cdc28 and cdc2, respectively.[6] With its cyclin partners, Cdk1 forms complexes that phosphorylate a variety of target substrates (over 75 have been identified in budding yeast); phosphorylation of these proteins leads to cell cycle progression.[7]

CDK1
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesCDK1, CDC2, CDC28A, P34CDC2, cyclin-dependent kinase 1, cyclin dependent kinase 1
External IDsOMIM: 116940; MGI: 88351; HomoloGene: 68203; GeneCards: CDK1; OMA:CDK1 - orthologs
Orthologs
SpeciesHumanMouse
Entrez

983

12534

Ensembl

ENSG00000170312

ENSMUSG00000019942

UniProt

P06493

P11440

RefSeq (mRNA)

NM_007659

RefSeq (protein)

NP_001163877
NP_001163878
NP_001307847
NP_001777
NP_203698

NP_031685

Location (UCSC)Chr 10: 60.78 – 60.79 MbChr 10: 69.17 – 69.19 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Structure

edit
 
Crystal Structure of the human Cdk1 homolog, Cdk2

Cdk1 is a small protein (approximately 34 kilodaltons), and is highly conserved. The human homolog of Cdk1, CDK1, shares approximately 63% amino-acid identity with its yeast homolog. Furthermore, human CDK1 is capable of rescuing fission yeast carrying a cdc2 mutation.[8][9] Cdk1 is comprised mostly by the bare protein kinase motif, which other protein kinases share. Cdk1, like other kinases, contains a cleft in which ATP fits. Substrates of Cdk1 bind near the mouth of the cleft, and Cdk1 residues catalyze the covalent bonding of the γ-phosphate to the oxygen of the hydroxyl serine/threonine of the substrate.

In addition to this catalytic core, Cdk1, like other cyclin-dependent kinases, contains a T-loop, which, in the absence of an interacting cyclin, prevents substrate binding to the Cdk1 active site. Cdk1 also contains a PSTAIRE helix, which, upon cyclin binding, moves and rearranges the active site, facilitating Cdk1 kinase activities.[10]

Function

edit
 
Fig. 1 The diagram shows the role of Cdk1 in progression through the S. cerevisiae cell cycle. Cln3-Cdk1 leads to Cln1,2-Cdk1 activity, eventually resulting in Clb5,6-Cdk1 activity and then Clb1-4-Cdk1 activity.[5]

When bound to its cyclin partners, Cdk1 phosphorylation leads to cell cycle progression. Cdk1 activity is best understood in S. cerevisiae, so Cdk1 S. cerevisiae activity is described here.

In the budding yeast, initial cell cycle entry is controlled by two regulatory complexes, SBF (SCB-binding factor) and MBF (MCB-binding factor). These two complexes control G1/S gene transcription; however, they are normally inactive. SBF is inhibited by the protein Whi5; however, when phosphorylated by Cln3-Cdk1, Whi5 is ejected from the nucleus, allowing for transcription of the G1/S regulon, which includes the G1/S cyclins Cln1,2.[11] G1/S cyclin-Cdk1 activity leads to preparation for S phase entry (e.g., duplication of centromeres or the spindle pole body), and a rise in the S cyclins (Clb5,6 in S. cerevisiae). Clb5,6-Cdk1 complexes directly lead to replication origin initiation;[12] however, they are inhibited by Sic1, preventing premature S phase initiation.

Cln1,2 and/or Clb5,6-Cdk1 complex activity leads to a sudden drop in Sic1 levels, allowing for coherent S phase entry. Finally, phosphorylation by M cyclins (e.g., Clb1, 2, 3 and 4) in complex with Cdk1 leads to spindle assembly and sister chromatid alignment. Cdk1 phosphorylation also leads to the activation of the ubiquitin-protein ligase APCCdc20, an activation which allows for chromatid segregation and, furthermore, degradation of M-phase cyclins. This destruction of M cyclins leads to the final events of mitosis (e.g., spindle disassembly, mitotic exit).

Regulation

edit

Given its essential role in cell cycle progression, Cdk1 is highly regulated. Most obviously, Cdk1 is regulated by its binding with its cyclin partners. Cyclin binding alters access to the active site of Cdk1, allowing for Cdk1 activity; furthermore, cyclins impart specificity to Cdk1 activity. At least some cyclins contain a hydrophobic patch which may directly interact with substrates, conferring target specificity.[13] Furthermore, cyclins can target Cdk1 to particular subcellular locations.

In addition to regulation by cyclins, Cdk1 is regulated by phosphorylation. A conserved tyrosine (Tyr15 in humans) leads to inhibition of Cdk1; this phosphorylation is thought to alter ATP orientation, preventing efficient kinase activity. In S. pombe, for example, incomplete DNA synthesis may lead to stabilization of this phosphorylation, preventing mitotic progression.[14] Wee1, conserved among all eukaryotes phosphorylates Tyr15, whereas members of the Cdc25 family are phosphatases, counteracting this activity. The balance between the two is thought to help govern cell cycle progression. Wee1 is controlled upstream by Cdr1, Cdr2, and Pom1.

Cdk1-cyclin complexes are also governed by direct binding of Cdk inhibitor proteins (CKIs). One such protein, already discussed, is Sic1. Sic1 is a stoichiometric inhibitor that binds directly to Clb5,6-Cdk1 complexes. Multisite phosphorylation, by Cdk1-Cln1/2, of Sic1 is thought to time Sic1 ubiquitination and destruction, and by extension, the timing of S-phase entry. Only until Sic1 inhibition is overcome can Clb5,6 activity occur and S phase initiation may begin.

Interactions

edit

Cdk1 has been shown to interact with:

See also

edit

Mastl

References

edit
  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000170312 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000019942 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ a b Morgan, David L. (2007). The cell cycle: principles of control. London: New Science Press. pp. 30–31. ISBN 978-0-19-920610-0.
  6. ^ Nasmyth K (April 1993). "Control of the yeast cell cycle by the Cdc28 protein kinase". Current Opinion in Cell Biology. 5 (2): 166–179. doi:10.1016/0955-0674(93)90099-C. PMID 8507488.
  7. ^ Enserink JM, Kolodner RD (May 2010). "An overview of Cdk1-controlled targets and processes". Cell Division. 5 (11): 11. doi:10.1186/1747-1028-5-11. PMC 2876151. PMID 20465793.
  8. ^ Lee MG, Nurse P (Jun 1987). "Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2". Nature. 327 (6117): 31–35. Bibcode:1987Natur.327...31L. doi:10.1038/327031a0. PMID 3553962. S2CID 4300190.
  9. ^ De Bondt HL, Rosenblatt J, Jancarik J, Jones HD, Morgan DO, Kim SH (June 1993). "Crystal structure of cyclin-dependent kinase 2". Nature. 363 (6430): 595–602. Bibcode:1993Natur.363..595D. doi:10.1038/363595a0. PMID 8510751. S2CID 4354370.
  10. ^ Jeffrey PD, Russo AA, Polyak K, Gibbs E, Hurwitz J, Massagué J, et al. (July 1995). "Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex". Nature. 376 (6538): 313–320. Bibcode:1995Natur.376..313J. doi:10.1038/376313a0. PMID 7630397. S2CID 4361179.
  11. ^ Skotheim JM, Di Talia S, Siggia ED, Cross FR (July 2008). "Positive feedback of G1 cyclins ensures coherent cell cycle entry". Nature. 454 (7202): 291–296. Bibcode:2008Natur.454..291S. doi:10.1038/nature07118. PMC 2606905. PMID 18633409.
  12. ^ Cross FR, Yuste-Rojas M, Gray S, Jacobson MD (July 1999). "Specialization and targeting of B-type cyclins". Molecular Cell. 4 (1): 11–19. doi:10.1016/S1097-2765(00)80183-5. PMID 10445023.
  13. ^ Brown NR, Noble ME, Endicott JA, Johnson LN (November 1999). "The structural basis for specificity of substrate and recruitment peptides for cyclin-dependent kinases". Nature Cell Biology. 1 (7): 438–443. doi:10.1038/15674. PMID 10559988. S2CID 17988582.
  14. ^ Elledge SJ (December 1996). "Cell cycle checkpoints: preventing an identity crisis". Science. 274 (5293): 1664–1672. Bibcode:1996Sci...274.1664E. doi:10.1126/science.274.5293.1664. PMID 8939848. S2CID 39235426.
  15. ^ Pathan N, Aime-Sempe C, Kitada S, Basu A, Haldar S, Reed JC (2001). "Microtubule-targeting drugs induce bcl-2 phosphorylation and association with Pin1". Neoplasia. 3 (6): 550–559. doi:10.1038/sj.neo.7900213. PMC 1506558. PMID 11774038.
  16. ^ Pathan N, Aime-Sempe C, Kitada S, Haldar S, Reed JC (2001). "Microtubule-targeting drugs induce Bcl-2 phosphorylation and association with Pin1". Neoplasia. 3 (1): 70–79. doi:10.1038/sj.neo.7900131. PMC 1505024. PMID 11326318.
  17. ^ a b Shanahan F, Seghezzi W, Parry D, Mahony D, Lees E (February 1999). "Cyclin E associates with BAF155 and BRG1, components of the mammalian SWI-SNF complex, and alters the ability of BRG1 to induce growth arrest". Molecular and Cellular Biology. 19 (2): 1460–1469. doi:10.1128/mcb.19.2.1460. PMC 116074. PMID 9891079.
  18. ^ Pines J, Hunter T (September 1989). "Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2". Cell. 58 (5): 833–846. doi:10.1016/0092-8674(89)90936-7. PMID 2570636. S2CID 20336733.
  19. ^ Kong M, Barnes EA, Ollendorff V, Donoghue DJ (March 2000). "Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction". The EMBO Journal. 19 (6): 1378–1388. doi:10.1093/emboj/19.6.1378. PMC 305678. PMID 10716937.
  20. ^ Koff A, Giordano A, Desai D, Yamashita K, Harper JW, Elledge S, et al. (September 1992). "Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle". Science. 257 (5077): 1689–1694. Bibcode:1992Sci...257.1689K. doi:10.1126/science.1388288. PMID 1388288.
  21. ^ Hannon GJ, Casso D, Beach D (March 1994). "KAP: a dual specificity phosphatase that interacts with cyclin-dependent kinases". Proceedings of the National Academy of Sciences of the United States of America. 91 (5): 1731–1735. Bibcode:1994PNAS...91.1731H. doi:10.1073/pnas.91.5.1731. PMC 43237. PMID 8127873.
  22. ^ Gyuris J, Golemis E, Chertkov H, Brent R (November 1993). "Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2". Cell. 75 (4): 791–803. doi:10.1016/0092-8674(93)90498-F. PMID 8242750.
  23. ^ He J, Xu J, Xu XX, Hall RA (July 2003). "Cell cycle-dependent phosphorylation of Disabled-2 by cdc2". Oncogene. 22 (29): 4524–4530. doi:10.1038/sj.onc.1206767. PMID 12881709.
  24. ^ Reuter TY, Medhurst AL, Waisfisz Q, Zhi Y, Herterich S, Hoehn H, et al. (October 2003). "Yeast two-hybrid screens imply involvement of Fanconi anemia proteins in transcription regulation, cell signaling, oxidative metabolism, and cellular transport". Experimental Cell Research. 289 (2): 211–221. doi:10.1016/S0014-4827(03)00261-1. PMID 14499622.
  25. ^ Kupfer GM, Yamashita T, Naf D, Suliman A, Asano S, D'Andrea AD (August 1997). "The Fanconi anemia polypeptide, FAC, binds to the cyclin-dependent kinase, cdc2". Blood. 90 (3): 1047–1054. doi:10.1182/blood.V90.3.1047. PMID 9242535.
  26. ^ Zhan Q, Antinore MJ, Wang XW, Carrier F, Smith ML, Harris CC, et al. (May 1999). "Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45". Oncogene. 18 (18): 2892–2900. doi:10.1038/sj.onc.1202667. PMID 10362260.
  27. ^ Jin S, Antinore MJ, Lung FD, Dong X, Zhao H, Fan F, et al. (June 2000). "The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression". The Journal of Biological Chemistry. 275 (22): 16602–16608. doi:10.1074/jbc.M000284200. PMID 10747892.
  28. ^ Yang Q, Manicone A, Coursen JD, Linke SP, Nagashima M, Forgues M, et al. (November 2000). "Identification of a functional domain in a GADD45-mediated G2/M checkpoint". The Journal of Biological Chemistry. 275 (47): 36892–36898. doi:10.1074/jbc.M005319200. PMID 10973963.
  29. ^ Vairapandi M, Balliet AG, Hoffman B, Liebermann DA (September 2002). "GADD45b and GADD45g are cdc2/cyclinB1 kinase inhibitors with a role in S and G2/M cell cycle checkpoints induced by genotoxic stress". Journal of Cellular Physiology. 192 (3): 327–338. doi:10.1002/jcp.10140. PMID 12124778. S2CID 19138273.
  30. ^ Tao W, Zhang S, Turenchalk GS, Stewart RA, St John MA, Chen W, et al. (February 1999). "Human homologue of the Drosophila melanogaster lats tumour suppressor modulates CDC2 activity". Nature Genetics. 21 (2): 177–181. doi:10.1038/5960. PMID 9988268. S2CID 32090556.
  31. ^ Kharbanda S, Yuan ZM, Rubin E, Weichselbaum R, Kufe D (August 1994). "Activation of Src-like p56/p53lyn tyrosine kinase by ionizing radiation". The Journal of Biological Chemistry. 269 (32): 20739–20743. doi:10.1016/S0021-9258(17)32054-9. PMID 8051175.
  32. ^ Pathan NI, Geahlen RL, Harrison ML (November 1996). "The protein-tyrosine kinase Lck associates with and is phosphorylated by Cdc2". The Journal of Biological Chemistry. 271 (44): 27517–27523. doi:10.1074/jbc.271.44.27517. PMID 8910336.
  33. ^ Luciani MG, Hutchins JR, Zheleva D, Hupp TR (July 2000). "The C-terminal regulatory domain of p53 contains a functional docking site for cyclin A". Journal of Molecular Biology. 300 (3): 503–518. doi:10.1006/jmbi.2000.3830. PMID 10884347.
  34. ^ Ababneh M, Götz C, Montenarh M (May 2001). "Downregulation of the cdc2/cyclin B protein kinase activity by binding of p53 to p34(cdc2)". Biochemical and Biophysical Research Communications. 283 (2): 507–512. doi:10.1006/bbrc.2001.4792. PMID 11327730.
  35. ^ Tan F, Lu L, Cai Y, Wang J, Xie Y, Wang L, et al. (July 2008). "Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells". Proteomics. 8 (14): 2885–2896. doi:10.1002/pmic.200700887. PMID 18655026. S2CID 25586938.

Further reading

edit
edit
  • Overview of all the structural information available in the PDB for UniProt: P06493 (Cyclin-dependent kinase 1) at the PDBe-KB.
Retrieved from "https://en.wikipedia.org/w/index.php?title=Cyclin-dependent_kinase_1&oldid=1242913512"